Remotely controlled drug delivery with chemical micro-robots
Room 3A07
Time: 10:00-11:00
Prof. Frantisek Stepanek
University of Chemistry and Technology, Prague, Czech Republic
http://www.vscht.cz/chobotix
The ability to control the rates of chemical or biochemical reactions at length-scales comparable to those of single-cell organisms or their sub-cellular compartments would be desirable in a number of situations, ranging from fundamental studies of transfer phenomena to applications such as controlled delivery of actives from functional materials in the pharmaceutical, food, or personal care products. Strategies for the control of biochemical reactions have been applied to autonomous micro-scale systems called “chemical robots”. A chemical robot is an internally structured microparticle consisting of a semi-permeable membrane that regulates molecular transport between the interior and the surroundings, a system of stimuli-responsive internal compartments that store and release reactants, immobilised enzymes or catalysts for facilitating chemical reactions, and magnetic nanoparticles that act as susceptors and enable receiving radiofrequency signals.
The talk will present an implementation of chemical robots based on soft hydrogel bodies produced by drop-on-demand inkjet printing and microfluidic methods, with internal storage reservoirs formed by phospholipid vesicles (liposomes) and immobilised enzyme as a bio-catalyst. Super-paramagnetic iron oxide nanoparticles that dissipate heat when exposed to alternating magnetic field in the radiofrequency range are used as susceptors that facilitate the control of local temperature, which in turn controls the diffusion rate of reactants from liposomes and thus the local reaction rate. The talk will cover fabrication methods for the bottom-up assembly of chemical robots, their structural and functional characterisation. Several scenarios will be presented, including simple one-off release of a pre-synthesised chemical payload, repeated on/off release, and finally repeated starting, stopping and restarting of a local chemical reaction that produces a chemically unstable but physiologically active product. The targeting of chemical robots to cancer cells (HT-29 cell line) using antigen-antibody interaction will be described. The flow and deposition of chemical robots in biological porous media both in vitro (3D cell cultures) and vivo (mouse models) will be presented.